Physiological and Biochemical Changes in the Seeds of Naturally Aged Wenling Medic (Medicago polymorpha) with Its Recovery of Viability

Wenling Medic (Medicago polymorpha) is common in southern China and has long been utilized as a vegetable eastern China, well significant raw ingredient for livestock pickled meals. As legume, there still research vacuum, the first problem faces production of seed germination. The germination percentage was low, according to practice laboratory tests performed this work. Furthermore, after more than two years storage, dropped sharply, it lacked enough life. An attempt made restore viability seeds using polyethylene glycol (PEG) osmoregulation-mediated priming method, physiological biochemical characteristics were assessed anthrone Bradford assay, 3,5-dinitrosalicylic acid (DNS) thiobarbituric (TBA) method. findings showed that relatively short storage age and, with useable life only under normal aging circumstances, not deactivated soon harvesting. In addition, whereas protein content α-amylase concentration did significantly correlate (p > 0.05) life, conductivity exudate, malondialdehyde content, carbohydrate < 0.05). considerably increased by saturation initiation mediated osmoregulation, utilizing PEG. conclusion, decrease seeds’ ability substantially connected higher levels lipid peroxidation decreased levels, but concentrations or activity. timing dehydration may need be carefully controlled when PEG osmoregulation prime seeds.